壳聚糖孵育低分子肝素钙脂质体的制备及其包封率测定

Preparation of Low Molecular Weight Heparin Calcium Liposome Incubated by Chitosan and Determination of Encapsulation Efficiency

目的:尝试通过壳聚糖孵育以提高低分子肝素钙脂质体包封率,并分析其可能机制。方法:采用薄膜分散法制备低分子肝素钙脂质体,1∶5壳聚糖溶液孵育低分子肝素钙脂质体。利用天青A法测定包封率。结果:低分子肝素钙脂质体经壳聚糖孵育前、后的包封率分别为(46.40±3.14)%、(97.94±1.33)%。透射电镜照片示镜下有纳米粒生成。结论:壳聚糖孵育的低分子肝素钙脂质体包封率大幅提高,可能是由于壳聚糖与游离的低分子肝素钙形成纳米粒所致。

OBJECTIVE： To improve the encapsulation efficiency fEE） of low molecular weight heparin （LMWH） calcium liposome through incubation with chitosan and analyze the possible mechanism. METHODS： The calcium liposome of LMWH was prepared by film dispersion method. Chitosan - incubated liposome was prepared by chitosan in a ratio of 1 ： 5. And the EE was determined by the method of Azure A. RESULTS： The EE of calcium liposome before incubation was （46.40±3.14） % as compared with （97.94±1.33）% after incubation with chitosan. Nanoparticle formation was noted under transmission electron microscope. CONCLUSION： The greatly enhanced EE of liposome after incubation with chitosan might be associated with the formation of nanoparticles from chitosan with free low molecular heparin calcium.