弱精症男子与正常人精子膜尿激酶型纤溶酶原激活因子含量的研究

Comparative quantification of the membrane-associated urokinase plasminogen activator (uPA) on the ejaculated spermatozoa between sterile patients with asthenospermia and healthy fertile Men

按ＷＨＯ精液参数标准，分别选择精子活力低下的男性不育症患者和正常生育力男子精液标本各２０例，将洗涤后的精子用含１％ＴｒｉｔｏｎＸ－１００的０．１ＭＴｒｉｓ－ＨＣｌ缓冲液处理，提取精子膜结合尿激酶型纤溶酶原激活因子（ｕＰＡ），然后采用抗人尿激酶多克隆抗体，进行各样品ｕＰＡ含量的测定（夹心法ＥＬＩＳＡ）。结果显示：弱精症男子精子膜结合ｕＰＡ（２３．１０±７．３５ｍｕ／１０６ｃｅｌｓ）显著性低于正常人精子膜ｕＰＡ（２９．８９±９．４８ｍｕ／１０６ｃｅｌｓ），Ｐ＜０．０２５。且精子膜ｕＰＡ含量与精子活率呈直线正相关（ｒ＝０．６４，Ｐ＜０．０００１），与精子活力（直线前向运动精子百分率）亦呈线性相关（ｒ＝０．４８，Ｐ＜０．００５）。说明精子膜结合ｕＰＡ可能与精子活力及生育力之间有一定关联。

Semens were selected from 20 cases of sterile patients with asthenospermia and healthy fertile men respectively according to the standards of WHO. Spermatozoa were washed and lysed with 0.1 M Tris-HCl buffer containing 1% Triton X-100. To quantify uPA in the samples, polyclonal antibodies against human urokinase were employed by means of a sandwich ELISA. In lysates of spermatozoa, significantly lower levels of uPA were found in patients with asthenospermia (23.1±7.35mu/10 6cells) as compared to healthy fertile men exhibiting normospermia (29.89±9.40mu/10 6cells). A positive correlation was observed between uPA quantity on spermatozoa and sperm viability ( r=0.64, P <0.0001), as well as with sperm motility ( r=0.48, P <0.005). Thus, it is inferred that membrane associated uPA on spermatozoa may be related to sperm motility and fertility.