小儿肺炎支原体感染的早期诊断

Early Diagnosis of Mycoplasma Pneumoniae Infection in Children

探讨小儿肺炎支原体感染的早期诊断方法。方法：对３８５例呼吸道感染的住院患儿应用聚合酶链反应（Ｐｏｌｙｍｅｒａｓｅｃｈａｉｎｒｅａｃｔｉｏｎ，ＰＣＲ）技术检测咽拭子肺炎支原体ＤＮＡ，同时行血冷凝集试验（Ｃｏｌｄａｇｇｌｕｔｉｎａｔｉｏｎｔｅｓｔ，ＣＡＴ），进行配对资料研究。结果：不同年龄患儿呼吸道感染肺炎支原体发病情况有所不同。ＰＣＲ法检测肺炎支原体ＤＮＡ阳性率不受自然病程的影响，ＣＡＴ法检测阳性率则明显受到病程的影响。ＰＣＲ法阳性率为３８．１８％，ＣＡＴ法阳性率为１１．６９％，差异有极显著性意义。结论：咽拭子ＰＣＲ法检测肺炎支原体ＤＮＡ，不受病程、年龄因素的影响，具有特异、快速、敏感、标本易于采集等优点。

Objective:To study an early diagnostic method for mycoplasma pneumoniae(MP) infection in childhood.Method:MP DNAs were determined by polymerase chain reaction(PCR) from nasopharyngeal swabs of 385 children with respiratory tract infection,and their serum samples were collected on the same day for cold agglutination test(CAT).Results:The positive rate of MPPCR was 38.18%,whereas that of serum CAT was 11.69%( P <0.001).The result of MP PCR was not related to the natural course of the respiratory tract infection,but the value of the serum CAT was associated with it.Conclusion:The MPPCR is a method with a good specificity and rapidity for early diagnosis of MP infection.