玉树组织培养中的器官形成与植株再生(英文)

Organ formation and plant regeneration in vitro tissue culture of Crassula arborescens

以玉树的茎、叶、根为外植体，研究不同培养基和植物生长调节剂对其再生的影响．结果表明，在附加0．1～10μmol·L^-1 NAA和10μmol·L^-1 BA的MS培养基上，不定芽形成率达到100％，每1个外植体再生芽数达到10个以上,将再生芽转接到舍有1μmol·L^-1 NAA或者1μmol·L^-1 IBA的1／2MS培养基上，发育成健壮小植株,生根植株移栽到大田，生长良好，8个月后株高达27cm．

Effects of medium and plant growth regulators on shoot regeneration from stem, leaf and root explants of Crassula arborescens were studied. Adventitious buds developed into shoots, shoot regeneration rate reached 100% and shoot number was above 10 per explant on the Murashige and Skoog basal medium （MS） supplemented with 0.1-10 μmol. L^-1 α-naphthaleneacetic acid （NAA） and 10 μmol. L^-1 N-6-benzyladenine （BA）. Rooted shoots grew into vigorous plantlets after transferred to 1/2 MS medium containing 1 μmol. L^-1 NAA or 1 μmol. L^-1 indolebutyric acid （IBA）. The rooted plantlets were transplanted to the field, grew very vigorous and reached 27 cm height after 8 months.