解脲支原体分群分型在妇女非淋菌性尿道炎诊断中的应用

Investigate on the infection of Ureaplasma urealyticum (Uu) in woman nongonococcal urethritis (NGU) population and its biovars and serotypes

目的探讨解脲支原体(Uu)分群分型在妇女非淋菌性尿道炎(NGU)诊断中的价值。方法采用PCR、DNA测序技术对Uu进行分群分型。结果经液体培养Uu阳性的177例中,采用PCR法检测Uu阳性为105例(59.3%),其中试验组(68例)生物1群感染54例(79.4%),生物2群感染13例(19.1%),生物1群和生物2群混合感染1例(1.5%),分布于14个血清型。结论Uu感染与寄居均以生物1群为主,而生物2群中的血清4型与妇女NGU发生具有显著相关。

[Objective] To investigate the infection of Ureaplasma urealyticum （Uu） in woman nongonococcal urethrifts （NGU） population and its biovars and serotypes. [Methods] The technique of culture isolation and molecular biology were used to study different serotypes of Uu infection in cervical specimens taken from NGU patients and gynecologic check-up persons in Shenzhen regions. [Results] 177 cases were screened to be Uu positive by liquid culture and 105 cases were found to be Uu positive by PCR method （59.3%）. In Uu positive 68 cases of the test group, 54 were Parvum biovar （79.4%）, 13 were Urealyficum biovar （19.1%） and the nfixed infections of these two biovars were 1 cases （1.5%）. [ Conclusion] The majority of Uu cervical colonization and pathogenesis was Parvum biovar, moreover, serotype 4 of Urealyficum biovar was the one most frequently found and strongly related with woman NGU.