动脉粥样硬化所致短暂性脑缺血发作及脑梗死患者血清E2选择素与一氧化氮水平的相关分析

Correlation between sE-selectin and NO in cerebral atherosclerosis

目的从可溶性E-选择素影响一氧化氮对血管壁保护作用的角度探讨脑动脉硬化的免疫病理机制。方法用ELISA方法测定40例脑梗死(IF)、34例短暂性脑缺血发作(TIA)及35例正常人的血清可溶性E-选择素(sE-选择素)水平,并采用硝酸还原酶法测定血清一氧化氮水平,分析它们之间的关系。结果TIA组及IF组患者血清sE-选择素的水平明显高于对照组(P<0.01),而一氧化氮的浓度明显低于对照组(P<0.01);并且IF组sE-选择素的水平明显高于TIA组(P<0.05),而一氧化氮的浓度明显低于TIA组。TIA组及IF组患者血清sE-选择素与一氧化氮变化呈明显的负相关(r=-0.692,P<0.01)。结论血清sE-选择素可能通过抑制一氧化氮的抗动脉硬化作用而在脑动脉硬化形成的过程中发挥了重要作用。

[Objective] To determine the effect of sE-selectins on NO in the immunopathogenesis of cerebral atherosclerosis. [Methods] The levels of sE-selectin in the serum of 40 cerebral infarction （IF）, 34 TIA patients and 35 healthy controls were measured with enzyme-linked immunosorbent assay （ELISA） and the level of NO was determined also. The correlation between sE-selectin and NO was analyzed. [Results] The serum levels of sE-selectin in IF and TIA group were significantly higher than those in healthy control（P 〈0.01 ） whereas the levels of NO were significantly lower（P 〈0.01 ）; moreover, the levels of sE-selectins in IF group were significantly higher than those in TIA group（P 〈0.05） whereas the levels of NO were significantly lower（P 〈0.05）. The level of sE-selectin was negatively correlated with the level of NO （r =-0.692, P 〈0.01）. [Conclusions] The increased levels of sE-selectins sup- press the levels of NO which has the protect effect to cerebral vascular endothelial cells. This suppression plays an important role in immunopathogenesis of cerebral atherosclerosis.