三种海洋微藻叶绿素a含量对抗生素胁迫的响应变化

Response of Chlorophyll-a Levels of Different Marine Microalgae to Antibiotic Stress

实验室条件下,研究不同抗生素胁迫对小球藻(Chlorella vulgarisBeij.)、金藻8701(Isochrysis galbana Parke8701.)和小新月菱形藻(Nitzschia closteriumEhr.)生长的影响。结果表明:1)低于100μg.mL-1的氯霉素作用下,小球藻和金藻8701叶绿素a含量变化与对照组无明显差异;小新月菱形藻叶绿素a含量显著降低。2)不同浓度梯度的遗传霉素(G418),均显著抑制3种微藻叶绿素a含量的增加。3)青霉素浓度低于100μg.mL-1时,能够促进3种藻叶绿素a的增加,但不同微藻间叶绿素a含量的相对增长率存在明显差异。试验结果可为微藻的基因工程选择标记和无菌培养体系的建立提供参考。

Three species of marine bait microalga. Chlorella. vulgaris, Isochrysis galbanastrain 8701, and Nitzschia closterium were subjected to different antibiotics. The microalga chlorophyll-levels were analyzed under laboratory conditions. Our results showed that ： 1） Chloramphenicol had no significant effect on the chloro-phyll-levels of C. vulgaris and I. galbanastrain 8701 when the concentration was〈100μg·mL^-1, but showed a significant inhibitory effect on N. closterium. 2） G418 significantly inhibited the growth of the three microalgae. 3） Penicillin could enhance the chlorophyll-a levels of the three species of marine bait microalga when its concentration was blow 100μg·mL^-1, but the relative growth rate （K） was remarkably different among these algae. The results of the study may be of reference to the axenic culture of microalgae and selective marker of marine microalgal genetic engineering.