线粒体肌酸激酶在食管癌组织中的表达

Expression of Mitochondrial Creatine Kinase in Human Esophageal Carcinoma

应用免疫组化ABC法，采用自制的兔抗人心肌线粒体肌酸激酶（CK－mimi）抗血清，对52例食管癌组织切片进行了检测。探讨食管癌组织中CK－mimi分布与临床病理分型的关系，旨在协助临床寻找食管癌新的诊断标志。结果显示：正常食管组织CK－mimi为阴性，52例食管癌全部为阳性，鳞癌随组织病理分级的增加而染色强度减弱。Ⅲ级鳞癌与Ⅰ级或Ⅱ级鳞癌相比，其减弱的程度均有统计学意义（P＜0．01）。提示CK－mimi可作为食管疾诊断、分级的免疫组化标志。对其防治研究具有一定的临床意义。

Paraffin sections of 52 cases of esophageal carcinoma were immunohistochemically stained with ABC technic using rabbit antibody aginst mitochondrialcreatine kinase of human heart. The results showed that the positive rate in esophagealsquamous cell carcinoma was 100%. The expression of CK-mimi in grade Ⅲ esophageal squamous cell carcinoma was lower than that in cases of grade Ⅰ and grade Ⅱ. Thestudy suggests that the positive granules of CK-miml may have some association with the histological types and that it may be used as a new tumor marker in the diagnosis ofesophageal carcinoma.