枳实提取物对实验性糖尿病小鼠肝脏抗氧化防御功能的影响

Effects of Citrus aurantium extract on liver antioxidant defense function in experimental diabetic mouse

目的研究枳实提取物对试验性糖尿病小鼠肝脏抗氧化能力的影响。方法用高、中、低剂量的枳实提取物治疗糖尿病小鼠5周后,观察其一般状况、肝脏的抗氧化能力及肝脏组织形态学变化。结果枳实提取物治疗组,与糖尿病模型组比较,血糖水平显著降低(P<0.05),谷胱甘肽含量(GSH)显著增加(P<0.05),谷胱甘肽过氧化物酶活性、丙二醛和NO含量显著降低(P<0.01),超过氧化物歧化酶活性有所增加。光镜下枳实提取物治疗组肝组织细胞损伤较糖尿病组降低。结论枳实提取物具有增强肝脏的抗氧化能力,降低肝细胞损伤作用。

Objective To study the effect of Citrus aurantium extract on liver antioxidant ability in experimental diabetic mice. Methods Experimental diabetic mice were treated with C. aurantium extract （ C. aurantium 5,10,15g/ kg） respectively. After 5 weeks, the general status, liver antioxidant ability and the change of livers histological were observed. Results Experimental diabetic mice groups were treated with C. aurantium extract in comparison with the experimental diabetic mice group. It was indicated that the levels of blood glucose were significantly reduced （ P 〈 0.05）, the levels of glutathione （GSH） were remarkably increased （P 〈 0.05 ）. The activities of glutathione peroxidase （GSHPx）, the contents of malon dialdehyde（MDA） and nitric oxide （NO） were significantly reduced （P〈 0.01 ）. The activities of superoxide dismutase （SOD） were increased in liver. The liver histological damages of experimental diabetic mice treated with C. aurantium extract were abated in comparison with the experimental diabetic mice under light microscope observation. Conclusion It was suggested that the extract of C. aurantium could effectively enhance the liver antioxidant function and decrease hepatocyte damages.