固相萃取富集净化-高效液相色谱串联质谱法测定饮用水中全氟化合物

A method of perfluorinated organic compounds in drinking water determinated by HPLC-MS/MS

目的建立固相萃取浓缩与高效液相色谱串联质谱法（HPLC-MS/MS）测定饮用水中全氟辛酸（PFOA）、全氟辛烷磺酸（PFOS）、全氟癸酸（PFDA）等全氟化合物的方法。方法样品经C18固相萃取浓缩净化,应用负离子电喷雾电离,检测方式为多级反应离子监测（MRM）模式,高效液相色谱串联质谱法（HPLC-MS/MS）进行测定。结果PFOA、PFOS、PFDA在50～1000pg/ml线性范围内,线性相关系数均〉0.99。在8、20.0pg/g添加水平的回收率为65%～111%,相对标准偏差为3.6%～14.6%（n=6）,定量检出限为8pg/g。结论该法适用于饮用水中PFOA、PFOS、PFDA等全氟化合物的测定,具有快速、定量准确、检测灵敏等优点。

Objective To establish determinated by HPLC-MS/MS. Methods a method of perfluorinated organic The contents of selected POCs （i compounds （POCs） in drinking water e. , perfluorooctanesuffonate （ PFOS ）, perfluorooctanoate （PFOA） and perfluorodecanoate （PFDA）） in water samples were extracted by the C18 solid-phase extraction （SPE）. The extraction samples were then identified and quantitated by high performance liquid chromatographynegative electrospray mass spectrometry （HPLC-ESI-MS/MS）. Results Linear calibration curves were obtained at the concentration ranges from 50pg/ml to 1000 pg/ml（the correlation coefficients were above 0.99）. The average recoveries for PFOA,PFOS and PFDA spiked in water ranged from 65 to 111% and their relative standard Were between 3.6% and 14.6% .The limit of quantification （LOQ） of the method was 8pg/g. Conclusion The method for determination of POCs in drinking water could be simple, sensitive and accurate.