不同浓度酒精摄入对小鼠脂质代谢及apoE表达的影响

Effects of alcohol on expressions of apoE in mice livers and brains

目的探讨不同浓度酒精摄入对小鼠载脂蛋白E((apoliprotein E,apoE)表达的影响,进一步阐明酒精摄入对apoE的作用效应。方法60只小鼠分为空白对照组、10%、30%和50%酒精浓度组,每组15只。8周后采血,生物化学方法测定相关血脂含量;并取其肝、脑组织,免疫组化检测apoE表达水平。结果各酒精处理组肝脏apoE表达均较空白对照组减弱,除10%酒精浓度组外,差别有统计学意义(P<0.05);apoE在脑中的表达则在各酒精浓度组间无统计学意义(P>0.05)。血清总胆固醇(TC)、甘油三脂(TG)和低密度脂蛋白胆固醇(LDL-C)较空白对照组增加,除10%酒精浓度组外,差别均有统计学意义(P<0.05);高密度脂蛋白胆固醇(HDL-C)则较空白对照组下降,差别有统计学意义(P<0.05)。结论长期较高浓度的酒精摄入可致小鼠肝apoE的表达减弱,并对血脂水平产生一定影响。

Objective To explore the effects of alcohol on expressions of apoE in mice livers and brains at different concentrations, and further to elucidate the effectiveness. Methods Sixty mice were randomly divided into the control group and 10% ,30% ,50% alcohol exposed groups （Fifteen mice in each group）. Blood samples were collected at the ninth week, then livers and brains were separated from mice. Expressions of apoE were detected by immunohistochemistry, and lipids were determined by biochemistry techniques. Results Compared with control group, the levers of apoE were significantly lower in the alcohol exposed groups except for the 10 % group （ P 〈 0.05 ）, and but no significant in brains （ P 〉 0.05）. TC, TG and LDL-C levels were significantly higher in the alcohol groups except for the 10% group than in the control group （P 〈 0.05）, and HDL-C were significantly lower in the alcohol exposed groups （P 〈 0.05）. Conclusion Long-term higher concentration alcohol intake decrease the expressions of apoE in livers and has an effect on lipids.