高效液相色谱法测定食品中脱氢乙酸的方法研究

Method study on dehydroaetic acid in food by high performance liquid chromatography

目的建立一种果汁、酱菜、腐乳等食品中脱氢乙酸防腐剂的高效液相色谱法。方法采用C18（5μm,250×4.60mm）色谱柱,流动相为甲醇-0.02mol/L乙酸铵（体积比为5∶95）,流速1.00ml/min;紫外检测波长306nm;柱温25℃;进样量10μl;外标法定量。结果方法所采用的标准曲线有良好的线性关系（r=0.9997）,方法的检出限为0.05mg/kg,加标回收率为96.5%～99.5%,对不同的样品基质相对标准偏差为1.09%～5.85%。结论该方法操作简便、准确,回收率高,精密度良好,重现性好,可用于果汁、酱菜、腐乳和月饼等食品中脱氢乙酸的测定。

Objective To estabish a high performance liquid chromatographic （HPLC） method for the determination of dehydroaetic acid in food determinated. Methods Separation was carried out on a column of C18 （5μm, 250 × 4.60mm） at the temperature of 25℃ with rnethanol/0.02mol/L ammonium acetate （5：95, V/V） as mobile phase, and elution flow rate was 1.0ml/min. The detection wavelength was 306nm and the injection volume was 10μl, quantitation was used by external standard method. Results The results showed a good linear relationship between the peak area and the concentration （r = 0.9997）, the recoveries for Dehydroaetic acid products were 96.5% - 99.5%, and the relative standard deviation was 1.09 % - 5.85 % for different kinds of food. Conclusion The results showed that the method may be simple, rapid, accurate and applicable for the determination of dehydroaetic acid in food.