反义碱性成纤维细胞生长因子Nudt6基因在小鼠内耳的表达

Expression of Anti-sense Basic Fibroblast Growth Factor(Nudt6) in the Murine Inner Ear

【目的】探讨反义碱性成纤维细胞生长因子Nudt6基因在小鼠内耳的表达。【方法】采用初生乳鼠内耳组织提取总RNA,逆转录后获得小鼠内耳细胞cDNA,根据基因编码区序列设计的引物进行PCR扩增,通过PCR产物分析和DNA测序确定Nudt6是否在小鼠内耳细胞表达。【结果】采用小鼠内耳组织总RNA,RT-PCR扩增出Nudt6基因部分编码区,扩增产物测序证实小鼠内耳中有Nudt6基因的表达。【结论】Nudt6基因在内耳有表达,为Nudt6与bFGF蛋白在内耳的互作关系提供了进一步的证据。Nudt6可能与bFGF在耳聋的预防、治疗中起作用。

[Objective]To ascertain the expression of anti-sense basic fibroblast growth factor（Nudt6） in the mouse cochlea.[Methods] Total RNA was extracted from the cochlea of 5 postnatal mice.After reverse transcription,resulting cDNA was amplified by PCR with primers targeted to nucleotide sequences corresponding to coding regions of Nudt6.The PCR products were sequenced to identify whether the Nudt6 was expressed in the mouse cochlea.[Results]After reverse transcription,resulting cDNA was amplified with one pairs of primers,and the products of Nudt6 was obtained.The expression of Nudt6 in the murine cochlea was confirmed by nucleotide sequencing.[Conclusion]Nudt6 is expressed in the murine cochlea.The results provide further evidence for the interaction of Nudt6 with bFGF.Nudt6 may play a role in prophylaxis,therapy of hearing loss by interacting with bFGF.