甲基强的松龙对多发性硬化患者PBMC细胞因子分泌的影响

Methylprednisolone Regulates the Cytokine Production of PBMC from Multiple Sclerosis Patients

【目的】多发性硬化(Multiple Sclerosis,MS)发病机制同T细胞异常活化介导的免疫紊乱密切相关。而Th1/Th2类细胞因子的失衡被认为积极参与了多发性硬化的病变过程。本文采集了多发性硬化病人在甲强龙冲击前后的外周血单核细胞(PBMC)进行体外培养,考察其在病情急性期及缓解期Th1/Th2类因子的分泌情况。【方法】收集多发性硬化病人急性期及缓解期外周血PBMC进行体外培养,应用ELISA法检测PBMC培养上清IFN-γ和IL-10含量,同对照健康患者进行比较,并对MS患者治疗前后进行自身对照比较。【结果】14名多发性硬化患者经甲强龙冲击后12名患者有明显或一定程度缓解,MS患者治疗前PBMC上清中IFN-γ含量显著高于对照组(P<0.05),治疗后MS患者同治疗前比较其PBMC分泌IFN-γ能力显著下降(P<0.05)。【结论】Th1类细胞因子积极参与了多发性硬化的发病过程,甲强龙冲击治疗后降低了Th1类细胞因子的分泌,使病情趋向缓解。

[Objeetive]The pathogenesis of Multiple Sclerosis（MS） is closely related to the abnormal activation of T cell and the imbalance of Th1/Th2 has been considered to be involved in. The PBMCs of MS patients before and after Methylprednisolone （MP） treatment were collected to detect the secretion of IFN-γ and IL-10 from PBMC. [Methods]PBMCs from MS patients at acute or remission stage were collected respectively, ELISA was adopted to detect IFN-γ and IL-10 in the supernatant of PBMC, and then the comparisons of cytokines between MS patients and control, and the comparisons between the same MS patients before and after MP treatment were made. [Results]After the MP treatment of all 14 MS patients, 12 of which evolved into remission stages, the IFN-γ of PBMC from MS patients before MP treatment was significantly higher than control （ P 〈0.05）, the IFN-γ of PBMC from MS patients after MP treatment was significantly lower than that of MS patients before MP treatment （ P 〈0.05）. [Conclusion]Th1 cyokines is actively involved in the pathogenesis of MS, and MP treatment reduces the Th1 secretion significantly which promotes the remission of MS.