P^(21)ras c-myc p53和雌孕激素受体在子宫内膜癌的表达

Expression of P 2 1 ras, c-myc, p53, estrogen receptor and progesterone receptor in endometrial carcinoma.

应用免疫组化ＬＳＡＢ法，对３２例子宫内膜癌，６例腺瘤型增生，２０例腺囊型增生组织的癌基因Ｐ２１ｒａｓ、ｃ－ｍｙｃ及抑癌基因ｐ５３蛋白表达进行了检测，对３２例子宫内膜癌进行了雌、孕激素受体的检测，并对子宫内膜癌组织Ｐ２１ｒａｓ、ｃ－ｍｙｃ、ｐ５３过度表达与雌、孕激素受体的关系作了分析。结果显示：Ｐ２１ｒａｓ、ｃ－ｍｙｃ、ｐ５３在子宫内膜癌组织中的阳性表达率分别为５９．４％、６２．５％和１８．８％；雌、孕激素受体的阳性率分别为７５．０％、７１．９％，Ｐ２１ｒａｓ阳性表达与组织分化及术后生存期有关；ｃ－ｍｙｃ阳性表达与子宫内膜癌患者临床预后无关；ｐ５３ｒａｓ阳性表达与组织学类型、组织分化程度及术后生存期有关，与孕激素受体呈负相关。Ｐ２１ｒａｓ阳性表达预示患者预后较好，突变型ｐ５３蛋白的出现与子宫内膜癌的恶性生物学行为有关。

Objective: To observe the expression rates of P 2 1ras, c-myc, p53 and investigate the relationship between expression of these genes and the expression of estrogen receptor (ER) and progesterone receptor (PR) in human endometrial carcinoma.Design: By using immunohistochemical staining on paraffin sections, we detected expression of P 2 1ras, c-myc, p53 proteins in 32 endometrial carcinomas, 6 adenomatous hyperplasia and 20 cystic glandalar hyperplasia, and expression of ER and PR were detected in 32 endometrial carcinomas.Results: Positive rates of expression of P 2 1, c-myc, p53, ER and PR in the 32 endometrial carcinomas were 59.4%, 62.5%, 18 8%, 75.0%, and 71.9%, respectively. In endometrial carcinoma, P 2 1ras expression was significantly associated with tumor grade and survival, and p53 expression was significantly associated with histologic type, tumor grade and survival. Expression of p53 was negatively correlated with PR. However, there was no correlation between expression of c-myc and the prognosis.Conclusion: Expression of P 2 1ras protein suggests better prognosis. An aberrant expression of p53 gene is indicative of aggressive biological behavior and poor survival in endometrial carcinomas. \ \