冰冻切片与石蜡切片中常见颅内肿瘤细胞的图像分析

IMAGE ANALYSIS OF TUMOR CELLS FROM COMMON INTRACRANIAL NEOPLASMS ON FROZEN AND PARAFFINED SLICES

应用图像分析技术对７６例常见颅内肿瘤定量分析，检测冰冻及石蜡切片时瘤细胞核四项形态参数。结果表明：冰冻切片中瘤细胞核面积、周长和直径均大于石蜡切片，而形状因子并无明显变化。随着星形细胞瘤的恶性程度增高，上述参数也有变化。本文研究结果将有助于颅内肿瘤冰冻切片的诊断。

Image analysis technique was used to measure four parameters [the form factor (FF), area, perimeter and diameter] of nuclei in 76 cases of common intracranial tumors on forzen and paraffined slices. The results showed that the area, perimeter, diameter of nuclei on frozen slices were much larger than those on paraffined slices while the FF showed no significant difference between two kinds of slices (P>0.05). The parameters of the area, perimeter, and diameter of nuclei on two kinds of slices became larger and the parameters of FF became smaller as the grade of astrocytoma ascended. It is considered that the results of present study will be helpful to the diagnosis of common intracranial tumors on frozen slices.