6株NDV鄱阳株对肝癌细胞杀伤作用的初步研究

Primary study on antitumor effect of six Newcastle disease virus strain Poyang on hepatocarcinoma cell

目的:研究从江西鄱阳湖野鸭分离的6株NDV对肝癌细胞的杀伤效应,进一步筛选NDV溶瘤毒株。方法:用MTT法研究6株NDV鄱阳株对两株传代肝癌细胞株Novikoff和HepG-2及一株正常肝细胞株HL-7702的杀伤效应。结果:6株NDV鄱阳株对肝癌细胞株Novikoff和HepG-2有显著杀伤效应,而对人正常肝细胞HL-7702无明显影响;病毒对细胞的杀伤活性与病毒作用剂量和病毒作用时间成正比;病毒在肝癌细胞中有明显复制增殖现象;Novikoff肝癌细胞对NDV敏感性强于HepG-2肝癌细胞。结论:6株NDV鄱阳株对Novikoff及HepG-2肝癌细胞产生显著的杀伤作用,而对正常肝细胞HL-7702未见明显影响。

Objective：Cytotoxicity assay of hepatocarcinoma cell treated with Poyang strains of Newcastle disease virus （NDV）. Methods： The cytotoxic effect of six NDV strains Poyang on the Novikoff ,HepG -2 and HL -7702 cell lines were investigated using MTT assay. Results： The apparently pathological changes were observed in Novikoff and HepG - 2 tumor cells, but not in HL - 7702 cells. The higher suppressed effect on Novikoff tumor cells than on HepG - 2 tumor cells in the NDV - strain Poyang. Conclusion ： NDV - strain Poyang can be a potential anticancer agent for the treatment of liver cancer.