雄黄抑制宫颈癌细胞株增殖和诱导凋亡的体外研究

The Study of Cervical Cancer Hela Cell inhibition and Apoptosis Induced by As4S4

目的:体外研究中药雄黄主要成分As4S4对子宫颈癌细胞株Hela细胞的增殖抑制和诱导凋亡的作用。方法:以As4S4不同浓度(7.5、15、30、60mg/L),分12h,24h,36h,48h,60h处理Hela细胞,用光镜观察细胞变化;四甲基偶氮唑蓝(MTT)法测定细胞增殖抑制率;流式细胞仪(FCM)检测细胞凋亡率;用DNA梯状电泳(DNA ladder)检测凋亡的发生。结果:不同浓度(7.5、15、306、0 mg/L)的As4S4处理的Hela细胞,细胞增殖受到抑制,作用呈明显的时效和量效关系,差异有非常显著性(P<0.01);As4S4诱导Hela细胞的凋亡率为(8.13±1.13)%^(62.36±4.42)%,与对照组比较(2.84±1.88)%,差异有极显著性(P<0.01),并呈浓度依赖性;DNA ladder呈梯状条带。结论:雄黄体外对宫颈癌细胞株Hela细胞具有增殖抑制和促进凋亡作用。

Objective： To investigate the Cervical cancer Hela cells growth inhibition and apoptosis induced by Tetra-arsenic tetra-sulfide （As4S4）. Methods： Hela cells were treated with As4S4 in various concentrations（7.5.15.30.60 mg/L）at different hours（ 12h, 24h, 36h, 48h, 60h）. Cell growth was measured by MTT. Apoptosis was detected by double staining flowcytometry （FCM） and DNA Ladder. Results： After treated with different concentrations of As4S4（7. 5, 15, 30, 60 mg/L ）, the cell growth of Hela was suppressed significantly with showed dose-and time-dependent manner increase in proliferative inhibition rate（P d0.01）. FCM analysis showed the As4S4 can induce apoptosis （8.13±1.13）% -（62.36 ±4.42）% significantly with showed dose-and time-dependent manner, compared with control group（2.84±1.88）% , （P〈 0. 01）; DNA fragmentation gel analysis showed DNA ladder. Conclusion： As4S4 could inhibit the proliferation and increase apoptosis in human Hela cells.