膀胱癌组织中MMP-2与TIMP-2的表达及相关性研究

Expression of Matrixmetalloproteinase-2 and Tissue Inhibitor of Metalloproteinase-2 in Transitional Cell Carcinoma of Bladder

目的:研究基质金属蛋白酶-2(MMP-2)和金属蛋白酶组织抑制因子-2(TIMP-2)在膀胱移行细胞癌组织中的表达及其与膀胱癌侵袭、转移的关系。方法:应用链霉菌抗生物素蛋白-过氧化酶(S-P)免疫组化方法,检测5例正常膀胱组织和35例膀胱移行细胞癌组织中MM P-2和TIMP-2的表达。结果:MMP-2、TIMP-2在膀胱正常组织中均呈阴性表达,而在35例膀胱癌组织中均不同程度的表达,阳性率分别为45.71%和51.43%。MMP-2随肿瘤病理分级和临床分期的增高而增高(P<0.05),TIMP-2的表达变化与肿瘤病理分级和临床分期的无相关性(P>0.05)。结论:MMP-2和TIMP-2相互作用对于膀胱癌的侵袭发展发挥了重要作用;MMP-2可能对判断膀胱癌的预后有帮助。

Objective： To study the expression of matrixmetalloproteinase-2（MMP-2） and tissue inhibitor of metalloproteinase-2（TIMP-2） in transitional cell carcinoma of bladder（TCCB） and their relationship to invasion and transition of the rumor. Methods： The expression of MMP-2 and TIMP-2 was analyzed immunohistochemically （SP method） in 5 normal bladder tissue and 35 patients with TCCB. Results： The expression of MMP-2 and TIMP-2 were negative in normal specimens of bladder, while among 35 cases, the expression rate of MMP-2 and TIMP-2 were 45. 71% and 51.43% respectively. The expression of MMP-2 increased with tumor stage and grade（P〈0. 05）, while there was no significant relationship between the expression of TIMP-2 and tumor stage and grade（P〉0. 05）. Conclusion： The interaction between MMP-2 and TIMP-2 play a key role in the invasion of TCCB, and MMP-2 may be useful in the evaluation of TCCB.