摘要
目的探讨儿童NK/T细胞淋巴瘤的免疫表型特征及其与EB病毒(EBV)感染的关系。方法NK/T细胞淋巴瘤5例标本,采用免疫组织化学链霉素抗生物素-过氧化酶连接法(SP法)检测CD45RO、CD3ε、CD56、CD20、T细胞内抗原(TIA-1)、丝氨酸蛋白酶-粒酶B(Granzyme B)确定肿瘤细胞免疫表型及EBV潜伏膜蛋白(LMP-1),原位杂交技术检测EBV编码的RNA(EBER1/2)。结果NK/T细胞淋巴瘤5例CD45RO、CD3ε、TIA-1和Granzyme B全部阳性,CD56阳性2例,CD20全部阴性。EBER1/2阳性4例,LMP-1阳性3例。结论EBV感染与儿童NK/T细胞淋巴瘤关系密切,EBV感染在儿童NK/T细胞淋巴瘤的发生发展中可能起重要作用。
Objective To study the immunophenotypic features of NK/T cell lymphoma in children and its association with Epstein-Barr virus （EBV） infection. Methods Five cases of children′s NK/T cell lymphoma were studied. CD45RO, CD3ε, CD56, CD20, TIA-1 and granzyme B were detected by immunohistochemistry staining for investigating immunophenotype. The expression of EBV-latent membrane protein （LMP-1） were detected by immunohistochemistry. EBV-encoded RNA （EBER1/2） were detected by in situ hybridization （ISH）. Results CD45RO, CD3ε, TIA-1 and granzyme B were positive in 5 cases, CD56 was positive in 2 cases, while CD20 negative in 5 cases.EBER1/2 positive in 4 cases and LMP-1 positive in 3 cases.Conclusions NK/T cell lymphoma in children is strong associated with EBV infection,and EBV infection may play an important role in the pathogenesis of children NK/T cell lymphoma.
出处
《实用儿科临床杂志》
CAS
CSCD
北大核心
2007年第3期178-179,共2页
Journal of Applied Clinical Pediatrics
基金
湖北省教育厅重点科研项目资助(B200624014)
