哮喘小鼠肺组织GATA-3及Th2型细胞因子表达的意义

Expressions of GATA-3 and Th2 Cytokines in Asthmatic Mice and Their Significances

目的探讨Th2特异性转录因子GATA-3及Th2型细胞因子(IL-5、13)在支气管哮喘(哮喘)小鼠中的表达及意义。方法BALB/c小鼠16只随机分为对照组和模型组。卵清蛋白致敏制备哮喘模型。HE染色观察小鼠肺组织病理变化,逆转录聚合酶链反应(RT-PCR)法检测小鼠肺组织GATA-3 mRNA表达,酶联免疫吸附法(ELISA)检测小鼠肺组织IL-5、-13蛋白的表达。结果HE染色示模型组支气管周围见大量炎性细胞浸润,而对照组则不明显。与对照组相比,模型组GATA-3 mRNA和IL-13蛋白表达量明显增加(Pa<0.05)。结论哮喘小鼠肺部存在GATA-3高表达,GATA-3可直接调控IL-5、-13表达,参与哮喘呼吸道炎症的发生。

Objective To investigate the expressions and significances of GATA-3 and Th2 cytokines in asthmatic mice.Methods Sixteen BALB/c mice were randomly divided into control group and asthmatic group.The pathological changes in trachial and pulmonary tissue were observed with HE staining method.The GATA-3 mRNA expression in pulmonary tissue was messured with reverse transcription polymerase chain reaction（RT-PCR）.And the IL-5,IL-13 proteins in pulmonary tissue were detected with enzyme linked immunosorbent assay（ELISA）.Results HE staining showed infiltration of a great deal of inflammatory cells around the bronchial wall in asthmatic group,while there was no obvious infiltration in control group,Compared with control group,the expressions of GATA-3 mRNA and IL-5,IL-13 protein in pulmonary tissue increased significantly（Pa〈0.05）.Conclusion GATA-3 expression increase in asthmatic mice,and GATA-3 can directly regulate the expressions of IL-5 and IL-13,and it may be involved in airway inflammation of asthma.