bFGF对人涎腺腺样囊性癌ACC-2细胞株增殖及MEK/ERK、NF-κB通路的影响

Effects of bFGF on the proliferation of human salivary adenoid cystic carcinoma ACC-2 cell line and MEK/ERK, NF-κB signaling pathway

目的:观察碱性成纤维细胞生长因子(basic fibroblast growth factor,bFGF)对人涎腺腺样囊性癌ACC-2细胞株增殖及MEK/ERK、核转录因子-κB(NF-κB)信号通路的影响。方法:培养人涎腺腺样囊性癌细胞株(ACC-2),MTT比色法测定不同浓度bFGF对细胞增殖的影响;免疫沉淀法纯化蛋白并ERK试剂盒测定ERK活性;Western-blot测定p-ERK及NF-κB抑制物(I-κBα)表达。并观察丝裂原蛋白活化激酶激酶(MEK)抑制剂U0126对上述指标的干预作用。结果:MTT实验显示bFGF明显增强ACC-2细胞增殖,免疫沉淀法显示bFGF上调ERK活性,免疫印记法显示bFGF明显增强p-ERK1/2表达及抑制I-κBα表达。U0126可抑制bFGF的以上效应。结论:bFGF可促进人涎腺腺样囊性癌ACC-2细胞株增殖,其途径与上调ERK活性,激活ERK、NF-κB信号通路有关。

Objective：To investigate the effects of bFGF on the proliferation of human salivary adenoid cystic carcinoma cell ACC-2 in vitro. Methods：The effect of bFGF on proliferation of ACC-2 cell line was observed by MTT assay; ERK activity was measured by immuno-precipitation ; and ERK and I-κBα expressions were assessed by Western blot. Results：bFGF can enhance the proliferation of ACC-2 cell line. Stimulated by bFGF, the proliferation ratio increased significantly. The intracellular ERK activity and p-ERK expression were increased and I- κBα expression was inhibited by different concentrations of bFGF. The above effects of bFGF can be attenuated by MEK inhibitor U0126. Conclusion：bFGF stimulates the proliferation of ACC-2 in the dose dependent manner, which may be due to up-regnlating ERK, NF-κB signaling pathway.