纳米羟基磷灰石根充糊剂动物根管充填及颌骨和皮下埋植实验

Reaction of experimental animial to a new nanohydroxyapatite in vivo

目的:评价新型纳米羟基磷灰石(n-HA)根充糊剂在动物体内的组织相容性。方法:以3只犬为实验对象,采用n-HA根充糊剂充填根管,颌骨埋植,皮下埋植,以AH-plus根充糊剂作对照,分别于术后2、4、12周处死动物,通过X线照片和病理切片观察材料对周围组织的影响。结果:①根充实验:X线照片显示试验牙根充严密,根尖周未见有明显的骨质破坏。实验2、4、12周组均未见根尖周不良改变,对照2周组根尖出现轻度炎性反应,4周组根尖周呈中、重度炎性反应,12周根尖周病变减轻,呈轻微炎性反应。②颌骨埋植:实验2、4、12周、对照12周组均未见炎性反应,对照2周组呈中度炎性反应,4周组呈轻微炎性反应。③皮下埋植:实验2周组、对照4周组有轻度炎细胞浸润,对照2周有中度炎性反应,其余各组病理切片均未见明显的炎性改变。结论:n-HA根充糊剂在动物体内的组织相容性明显好于AH-plus根充糊剂。

Objective： To assess the tissue reactions of dogs to a new nanohydroxyapatite. Methods：Three dogs were used for root canal filling experiment, mandibular implanting and subcutaneous implanting experiment, AH-plus root canal sealer was used as control. The dogs were killed after implantation for 2,4 and 12 weeks separately. The tissue reaction was assessed by X-ray and light microscope examination. Results：（1）Root canal filling experiment ： no inflammatory reaction was detected in specimens of n-HA group. In AH-plus group, implantation caused a middling inflammatory reaction after 2 weeks and 4 weeks. After 12 weeks the reaction of apical tissues was slight. （2）Mandibular implanting experiment： no inflammatory reaction was showed by all n- HA groups and the group of AH-plus implanted for 12 weeks. Mild inflammatory reaction was observed in the groups of AH-plus implanted for 2 weeks and 4 weeks. （3） Subcutaneous implanting experiment ： after AH-plus was implanted for 2 weeks and 4 weeks, middling inflammatory reaction was observed. The group of n-HA implanted for 2 weeks showed the same reaction. No obvious inflammatory reaction was found in the other groups, Conclusion：n-HA shows better tissue compatibility than AH-plus.