血清饥饿和低氧对口腔鳞癌细胞系血管内皮生长因子表达的影响

Serum-starvation and hypoxia regulation of expression of vascular endothelial growth factor in oral squamous cell carcinoma

目的:探讨血清饥饿和低氧对体外培养的口腔鳞癌细胞系血管内皮生长因子(VEGF)表达的调节。方法:采用半定量逆转录聚合酶链反应(RT-PCR)检测血清饥饿和缺氧条件下口腔鳞癌TSCCa细胞系和颈淋巴转移癌GNM细胞系中VEGF mRNA表达情况,同时用酶联免疫吸附实验(ELISA)的方法定量测定了2细胞系中VEGF的活性变化。结果:RT-PCR结果显示GNM细胞VEGF mRNA表达水平均较TSCCa细胞高(P<0.05),血清饥饿和低氧处理时在TSCCa细胞和GNM细胞中VEGF mRNA均有明显的增加,以TSCCa细胞系中VEGF mRNA增加的尤为明显。低氧处理4h时,VEGF的活性便显著增加,8h时达到最高。GNM细胞中VEGF增加至2倍,而TSCCa细胞中VEGF增加至6倍。血清饥饿组在TSCCa细胞中VEGF的活性增加至4倍,明显高于GNM细胞中的2倍。结论:血清饥饿和低氧能显著上调口腔鳞癌细胞VEGF的表达和活性,可能在口腔鳞癌血管形成中起重要作用。

Objective：To investigate the expression of vascular endothelial growth factor （VEGF） in oral squamous cell carcinoma by serum-starvation and hypoxia. Methods：The expression of VEGF mRNA was evaluated in two oral squamous cell carcinomas cells TSCCa and GNM treated with serum-starvation and hypoxia, using semiquantitative RT-PCR. The activities of VEGF in two cell lines were detected by enzyme linked immunosorbent assay （ELISA）. Results-Contrast to TSCCa cells, GNM cells expressed significantly higher levels of mRNA of VEGF by RT-PCR detection. Serum-starvation and hypoxia could increased expression of VEGF mRNA in both two cell lines in vitro, the levels of mRNA of VEGF increased more in TSCCa cell lines than that in GNM cell lines when treated with serum-starvation and hypoxic. After 4 hours hypoxic treatment, the activities of VEGF increased significantly and approached the peak after 8 hours ; the activities of VEGF in GNM cell lines was dramatically twice higher than that of the control group, while the activities of VEGF increased by 6 times in TSCCa cell lines. The activities of VEGF in TSCCa cell lines were higher than that of in GNM cell lines when treated with serum-starvation. Condusion： Serum-starvation and hypoxia can up-regulate VEGF mRNA expression and activities in oral squamous cell lines in vitro ,which may play an important role in the angiogenesis of oral squamous cell carcinoma.