椎板切除碎骨粒回植法制作腰椎管狭窄动物模型

The manufacture of animal model of lumbar spinal stenosis adopting laminectomy and bone particles replanting in-situ

目的:探索一种制作腰椎管狭窄动物模型的简便有效的方法。方法:以SD大鼠为模型,分别行L5全椎板切除(B组)及切除后自体碎骨粒回植(A组),与空白对照组(C组)一同进行下肢运动功能(BBB评分及出现间歇性跛行前的行走距离)和体表诱发电位(SEP)检查以观察腰椎管狭窄的表现,并进行L5的影像学及病理学检查以观察和测量腰椎管狭窄的程度。结果进行统计学分析以检验模型对腰椎管狭窄模拟的效果。结果:A、B组术后均出现了明显的椎板再生和腰椎管狭窄的表现,且以A组更为明显。A、B组BBB评分分别自术后2周和1周恢复正常(P>0.05)。术后16周时,在3组大鼠中A组出现间歇性跛行前的行走距离最短(P<0.05),其均数较C组减少42.9%,B组较C组减少17.1%(P<0.05)。A、B组术后SEP潜伏期与时间呈正相关(P<0.05),波幅与时间呈负相关(P<0.05);术后16周时A、B组潜伏期均数分别较C组延长1.4倍和0.75倍,波幅均数降低78.9%和56.3%,且与C组相比,A、B组以上变化均有显著的统计学差异(P<0.05),其中A组最为明显(P<0.05)。术后16周时病理显示A组L5椎管狭窄最为严重(P<0.05),截面积较C组减少34.1%,B组较C组减少18.2%(P<0.05),L5的X线、CT和MR检查亦显示A、B组椎板再生,椎管狭窄。结论:椎板切除碎骨粒回植法制作腰椎管狭窄动物模型的效果显著,模拟性良好,是制作该类动物模型的简便有效的方法。

Objective： To explore a simple and effective method for making animal models of lumbar spinal stenosis. Mothods： SD rats were selected and divided： group A received autogeneic bone transplantation in-situ after L5 laminectomy, group B performed only laminectomy on Ls, All rats including control （group C） were evaluated by examining motor functions （BBB score and walking distance before the intermittent claudication happened） and SEP to analyze the presentation of stenosis, as well radiological and pathological examining to observe and measure the degree of stenosis. The data was analyzed statistically. Restuhs： Both group A and B showed bone regeneration and stenosis obviously, especially in group A. The BBB scores of group A and B recovered to normal level 2 and 1 week after the operation （P〉0.05）. Animals in group A had the shortest walking distance （P〈0.05）before the occurring of intermittent claudication 16 weeks after the operation, with the decrease of 42.9%, and group B 17.1% comparing with group C （P〈0.05）. The SEP latent period of group A and B correlated with time positively while the amplitude negatively（ P〈0.05 ）. 16 weeks after operation, the mean latdnt period of group A and B increased about 1.4 and 0.75 time while the mean amplitude decreased about 78.9% and 56.3% compared with group C. Besides, cross-sectional area of L5 vertebral canal of group A was the smallest （P〈0.05）among 3 groups, decreasing about 34.1%, and group B 18.2% compared with that of group C （ P〈0.05 ）according to the pathology, meanwhile, the X-ray, CT and MR of L5 also showed bone regeneration and stenosis in group A and B. Condtmiona： The laminectomy and bone particles replanting in-situ is an easy and effective method to make animal model of lumbar spinal stenosis.