摘要
目的:用珍珠层/聚乳酸人工骨(N/P)复合同种异体成骨细胞(osteoblastsOBs)修复兔桡骨节段性骨缺损,并从放射学角度评价成骨效果。方法:手术制作18只新西兰大白兔15mm双侧桡骨缺损模型;体外培养同种异体成骨细胞,分别种植到珍珠层/聚乳酸人工骨和聚乳酸(PLA)材料上。以复合OBs的珍珠层/聚乳酸人工骨为实验组(ON),以复合OBs的聚乳酸(OP)和未复合OBs的珍珠层/聚乳酸人工骨(N)作对照组,移植修复兔桡骨缺损。分别于植入后4、8、12周取材,经大体观察、X线、放射学评分和X线阻射密度值来评价修复兔桡骨节段性骨缺损的效果并进行统计学分析。结果:复合OBs的N/P人工骨的试验组放射学评分和X线阻射密度值均高于对照组,统计学差异有显著性。在新骨形成的同时,珍珠层粉未完全降解。结论:珍珠层人工骨能较好地修复兔桡骨骨缺损,在复合成骨细胞后修复效果更佳,是一种很有前途的骨组织工程材料。
Objective： To evaluate the osteogenesis of nacre artificial bone combined with allogeneic osteoblasts in repairing rabbit radius segmental defect. Methods： The defect with the length of 15mm was produced on bilateral radii of 18 adult New Zealand white rabbits by surgery. Osteoblasts seeded into nacre artificial bone and PLA were co-cultured in vitro, and then implanted into defect sites of model animals. Nacre artificial bone without osteoblasts was served as a control. The repairing effect was evaluated at 4,8,12 weeks post-operation respectively by gross observation, radiograph examination, radiographic scores, optical density scores and then analyzed in statistics. Results： The radiographic and optical density scores in group of nacre artificial bone combined with osteoblasts were higher than those in group of PLA combined with osteoblast and group of nacre artificial bone, and there was statistic significance among them. However, a part of nacre powder degraded during the process of new bone formation. Conclusions： Nacre artificial bone is reliable to repair rabbit radius defect. Whereas, osteogenesis of it is more effective after combining with osteoblasts.
出处
《中国临床解剖学杂志》
CSCD
北大核心
2007年第6期673-676,共4页
Chinese Journal of Clinical Anatomy
基金
国家自然科学基金项目(30271321)
