摘要
目的:比较分析transwell和划痕法(Wound healing)检测细胞迁移能力的差异,探讨两种方法在血清饥饿调节的血管平滑肌细胞迁移中的应用。方法:用DMEM和含10%小牛血清的DMEM分别作用于体外培养的大鼠血管平滑肌细胞24h,采用transwell迁移小室和划痕方法检测血清饥饿(serum starvation)和有血清条件下平滑肌细胞的迁移能力。结果:transwell法和划痕法分别显示了VSMC在血清饥饿组的迁移数目和距离明显比10%血清刺激组少和短。结论:两种方法都能较好地反映细胞的迁移能力,即血清饥饿组明显低于10%血清刺激组。但划痕法更适用于本实验。
Objective： To investigate the difference between Transwell assay and Wound healing assay for detecting serum starvation-regulated migration of vascular smooth muscle cell （VSMC）, Methods： VSMCs were isloated fi＇om the male Sprague-Dawley rats adopting the explant method. Effects of serum flee culture on the migration of VSMC were detected by Transwell assay and Wound healing assay respectively. Results： The migration ability of VSMC was significantly inhibited by serum starvation compared with 10% FCS/DMEM stimulation. Conclusions： Both of two methods could be used to examine the migration of VSMC under serum starvation condition and Wound healing assay was more suitable to be chosen,
出处
《中国临床解剖学杂志》
CSCD
北大核心
2007年第6期687-689,共3页
Chinese Journal of Clinical Anatomy
