门静脉高压症猪门静脉的生物力学特性及其临床意义

The biomechanical properties of swine portal vein in portal hypertension and their clinical significance

目的:建立猪门静脉高压症模型,探讨门静脉高压症时门静脉的生物力学特性。方法:采用2月龄湖北白种猪,用四氯化碳、苯巴比妥、乙醇,配合高脂、低蛋白、低胆碱饮食进行混合饲养。通过脾静脉插管测压,取门静脉在生物软组织力学试验机上测定其压力-直径关系,横断取材,冰冻切片,H E法染色,用计算机图像分析系统测量其几何形态学指标。结果:实验组门静脉压为(4.17±1.03)kPa,对照组为(1.51±0.79)kPa(P<0.01),实验组门静脉的Einc、Ep和EV均随压力的上升而增大,在相同压力下明显大于对照组的Einc、Ep和EV。在0～4 kPa压力范围内实验组门静脉的顺应性(C)显著低于对照组,而在4～8 kPa的高压时两者顺应性差异并不明显(P>0.05)。结论:门静脉高压症时,门静脉的生物力学特性均发生了明显变化。肝移植时,移植材料间的生物力学特性也应考虑。

Objective： To explore the biomechanical properties of swine portal vein in portal hypertension. Methods： 2-months-old, white swines of Hubei were fed with the food of high grease, low protein and choline, carbon tetrachloride, fenobarbital and alcohol. Portal vein pressure was measured by puncturing spleen vein. Swine portal veins were removed and analyzed the pressure-diameter relationship on the biomechanical machine. The geometrical morphologic data of portal vein were obtained by the computer image analysis system after HE staining of portal vein tissues. Results： Portal vein pressure of experimental group was about （4.17±1.03）kPa, while control group（ 1.51±0.79 ）kPa（P〈0.01）. The Einc, Ep and Ev of swine portal vein increased with rising of pressure. However, at the same pressure, three kinds of elastic modulus of experimental group were higher than that of control group, the compliance of portal vein of experimental group were lower than that of control group. Conclusions： In portal hypertension, the geometry morphology and the biomechanical properties of swine portal vein were greatly changed.