急性肺损伤大鼠肺组织COX-2、NF-κB表达及其相关性的研究

Expression and Relativity of Cyclooxgenase-2 and Nuclear Factor κB in Rat Lung Tissue of Acute Lung Injury

【目的】观察肺组织NF-κB、COX-2表达及其相关性,探讨NF-κB在感染性急性肺损伤肺组织病理变化中的作用机制。【方法】采用静脉注射LPS诱导大鼠ALI模型,观察大鼠肺湿干重比及动脉血气分析变化;HE染色光镜下了解肺组织病理改变;免疫组织化学法检测肺组织COX-2、NF-κB p65的表达。【结果】急性肺损伤组大鼠氧分压明显下降,存在不同程度的酸血症及过度通气,肺湿干重比明显增加(P<0.05)。正常大鼠肺组织表达少量COX-2,NF-κB p65仅在胞浆表达,急性肺损伤时大鼠肺组织中COX-2大量表达,同时NF-κB p65表达由细胞浆转移到细胞核,且两者呈高度相关(r=0.924)。【结论】急性肺损伤大鼠肺组织COX-2、NF-κB表达明显增加,且相关性高。

[Objective]To observe expression and relativity of cyclooxgenase-2 and NF-κB in rat lung tissue and discuss the mechanism of NF-κB in acute lung injury. [Methods]The SD rats were injected lipopolysaccharide to establish the model of acute lung injury. Blood gas and wet/dry weight ratio （W/D） of lung were measured. The expression of cyclooxgenase-2 and nuclear factorκB were measured by immunohistochemical method, [Results]The PaO2 of rats in acute lung injury group significantly decreased compared with natural group. W/D increased in acute lung injury group（ P 〈0.05）. Cyclooxgenase-2 and nuclear factorκB expressed a little in plasm in normal rats lung. But cyclooxgenase-2 expressed massively in rats lung tissue of acute lung injury. at the same time, the expression of nuclear factor κB transfered from plasm to nucleus. The correlation between them were high（r=0. 924）. [Conclusion]The expression of cyclooxgenase-2 and nuclear factorκB significantly increases in acute lung injury. They are highly correlated.