胃癌组织TAM、MC和LV计数及其临床病理意义

Counts of Tumor-associated Macrophage(TAM),Mast cell(MC),Lymph Vessel(LV) and Their Clinicopathological Significances in Gastric Cancer Tissues

【目的】研究胃癌组织中肿瘤相关巨噬细胞(TAM)、肥大细胞(MC)及淋巴管(LV)计数及相互关系和临床病理意义。【方法】60例胃癌组织常规制作石蜡包埋切片,TAM、MC和LV染色方法均为SP免疫组化法。【结果】60例胃癌TAM、MC和LV计数分别为18.4±5.6、15.2±4.3和13.5±4.8;高分化、侵袭肌层、无淋巴结转移及无远处转移病例TAM、MC、LV计数明显低于分化或未分化、侵袭浆膜层或浆膜外、淋巴结转移及远处转移病例(P<0.05或P<0.01);LV计数与TAM计数(r=0.43,P<0.01)及MC计数(r=0.38,P<0.01)均存在密切正相关,TAM计数与MC计数存在密切正相关(r=0.32,P<0.05)。【结论】TAM、MC和LV计数可能是反映胃癌进展、生物学行为和预后的重要标记物,TAM和MC可能具有促肿瘤淋巴管生成作用。

[Objective]To study the counts of TAM, MC, LV and detect their clinicopathological significances and correlations in gastric cancer tissues. [Methods]Spimmunohistochemical method was used for the counts of TAM,MC and LV on the routinely paraffin-embedded sections from 60 cases of gastric cancer. [Results]The mean count of TAM,MC and LV was 18.4±5.6,15.2±4.3 and 13.5±4.8 in gastric cancer tissues, respectively. The mean count of TAM, MC and LV was significantly lower in the cases of well-differentiation,infiltrating muscular layer, without-metastasis of lymphnode, and distant organs than those in the ones of poor or undifferentiation,infiltrating serous layer,with-metastasis of lymphnode ,and distant org-ans （ P 〈0. 05 or P 〈0.01）. The close positive correlations were found among the LV counts and TAM counts （r= 0.43, P 〈0.01）and MC counts （r= 0. 38, P 〈0. 01）. Also the close positive correlation was found between the counts of TAM and MC（r= 0. 32, P 〈0.05）. [Conclusion]The counts of TAM, MC or LV might be important markers for reflecting the progression,biological behaviors and prognosis of gastric cancer. TAM and （or） MC might have promoting effects on lymphangiogenesis of gastric cancer or other malignant lesions.