人端粒酶逆转录酶在膀胱肿瘤中的表达及其与预后的关系

Relationship between the expression of human telomerase reverse transcriptase and the prognosis in bladder neoplasm

目的检测人端粒酶逆转录酶(hTERT)在膀胱肿瘤中的定量表达,探讨hTERT与膀胱肿瘤预后复发的关系。方法采用免疫组织化学法和高清晰彩色医学图文分析系统,对45例膀胱移行细胞癌(BTCC)组织标本中的hTERT的表达情况进行定量检测,并用13例正常膀胱组织标本作为对照。结果BTCC组织标本中均可见有hTERT的表达,正常膀胱组织中无表达。hTERT的表达强度与患者的性别、年龄、肿瘤的大小、部位以及肿瘤的单多发等情况无关(P>0.05),而与肿瘤复发有关(P<0.05)。结论hTERT在膀胱肿瘤中表达上调,其活性与预后相关,可以作为检测肿瘤复发的指标。

Objective To detect the quantitative expression of human telomerase reverse transcriptase （hTERT） in bladder neoplasm, and to investigate the correlation between hTERT and prognosis. Methods The quantitative expression of hTERT was detected in 45 cases of bladder transitional cell carcinoma （ BTCC ） tissues and 13 cases of human normal bladder mucosa tissues （control group） with immunohistochemistry method and HMIAS-2000 image analysis system. Results The expression of hTERT in 45 cases of BTCC tissues was positive and in 13 cases of human normal bladder mucosa tissues was negative. The expression of hTERT was uncorrelated with the sex and age of patients, the size and position of tumor, single and multiple tumor （P 〉 0. 05） , but it was correlated with recurrence of BTCC （ P 〈 0. 05 ）. Conclusion The up-regulated expression of hTERT in BTCC suggests that, it is correlated with the prognosis and can be regarded as a marker for the recurrence.