子宫内膜异位症患者外周血和腹腔液中IFN-γ和IL-4的水平测定

Determination of IFN-γ and IL-4 in Peripheral Blood and Abdominal Fluid among the Patients with Endometriosis and its Relation

[目的]探讨子宫内膜异位症(内异症)患者外周血和腹腔液中的T辅助淋巴细胞(Th细胞)分泌干扰素γ(IFN-γ)和白细胞介素(IL-4)的水平。[方法]选取经术后病理证实为内异症的患者24例作为内异症组,以子宫肌瘤和卵巢良性肿瘤患者24例作为对照组。分别分离、培养各组外周血和腹腔液中的Th细胞,用酶联免疫吸附试验(ELISA)检测其培养的上清液中IFN-γ和IL-4的水平,并计算IFN-γ/IL-4的比值。[结果]内异症组外周血和腹腔液中Th细胞培养上清液IFN-γ的水平都显著低于对照组(P<0.01);IL-4的水平都显著高于对照组(P<0.05),IFN-γ/IL-4的比值都显著低于对照组(P<0.01)。[结论]内异症组患者外周血和腹腔液中Th细胞分泌IFN-γ的水平下降,而IL-4的水平上升,导致IFN-γ/IL-4比值降低,揭示Th1和Th2细胞比例失衡。这些变化导致子宫内膜异位种植,促进了内异症的发生。

[Objective] To explore the concentrations of interferon-γ （IFN-γ） and interleukin-4 （IL-4） secreted by T helper cell（Th cell） in the peripheral blood and fluid among patients. [Methods]24 patients with histopathological diagnosed endometriosis after operation were selected as endometriosis group, 24 patients with leiomyoma or benign ovarian tumor were selected as control group. Th cell in the peripheral blood and peritoneal fluid were isolated and cultured, the concentrations of IFN-γ and IL-4 in culture media were determined by enzyme-linked immunosorbent assay（ELISA）, and the ratio of IFN-y/IL-4 was calculate. [Results]The mean concentration of IFN-γ secreted by Th cell in peritoneal blood and abdominal fluid is both significantly lower than that in control group（ P〈0.01） ; The mean IL-4 is both higher than that in control group（ P〈0.05）. The ratio of IFN-γ/ IL-4 is both significantly lower than that in control group（ P〈0.01）. [Conclusion] The Th cell of endometriosis group secreting IFN-γ is declined but secreting IL-4 is significantly strengthened. Therefore,the decline of IFN-γ/ IL-4 suggests that there is unbalanced between Th1 and Th2. All these changes accelerate the beginning of endometriosis.