摘要
目的建立测定硝呋太尔片含量的高效液相色谱(HPLC)法。方法色谱柱为DiamonsilC18柱(250mm×4.6mm,5μm),流动相为甲醇-水(50∶50),流速为1.0mL/min,柱温为30℃,检测波长为260nm。结果硝呋太尔的质量浓度在50.15～300.9μg/mL范围内与峰面积线性关系良好(r=0.9999,n=6),平均加样回收率为101.2%,RSD=1.8%(n=6)。结论HPLC法简便易行、准确可靠,可作为硝呋太尔片的含量测定方法。
Objective To establish a HPLC method for the content determination of nifuratel tablet. Methods The determination was performed on Diamonsil C18 column under 30℃ ;the mobile phase consisted of methanol-water （50： 50） with the flow rate of 1.0 mL/min, the detecting wavelength at 260 nm. Results The calibration curve of nifuratel was linear from 50. 15-300.9 μg/mL （r=0. 999 9, n = 6）, the average recovery rate and relative standard deviation were 101.2% and 1.8% （n =6） respectively. Conclusion The method is rapid, simple,accurate and precise. It carl be used for the content determination of nifuratel tablet.
出处
《中国药业》
CAS
2007年第23期22-23,共2页
China Pharmaceuticals
