白虎汤对鸡毒支原体体外抑制作用及其超微结构的影响

Influence of Baihu Tang on morphology and ultrastructure of MGPG31

目的:观察鸡毒支原体(MGPG31)形态和超微结构变化,探讨白虎汤体外对MGPG31抑制作用的机理。方法:(1)用二倍稀释法测定白虎汤对MGPG31的MIC;(2)在MIC(0.094g/ml)和亚MIC(0.0235g/ml)的条件下取白虎汤与鸡毒支原体培养48h后的样品,通过透射电镜观察支原体的形态和超微结构变化。结果:白虎汤对鸡毒支原体的MIC1和MIC2分别为0.047g/ml和0.094g/ml。白虎汤主要作用于繁殖期支原体,影响细胞膜通透性和形态而起到抑制作用。

Objective： To study the mechanism of Baihu Tang that inhibiting mycoplast by observing the change of morphology and ultrastructure on MGPG31. Methods： MIC of Baihu Tang on MGPG31 was determined using double dilution method.Atfer 48 hours of cultivation with Baihu Tang at MIC （0. 094 g/ml） and Sub- MIC （0. 023 5g/ml） . MGP31＇ morphology and ultrastructure was observed under transmission electron microscope. Results： MIC and Sub -- MIC of Baihu Tang on MGPG31 was 0.094g/ml and 0. 047g/ml. It can inhibit the growth of mycoplas in the idiophase by changing the permeability and morphous of cell envelope.