反复热性惊厥大鼠体内促炎症细胞因子水平的变化

Inflammatory cytokines levels in rats with frequent febrile seizures

目的:研究反复热性惊厥(febrileseizures,FS)大鼠海马IL-1β、IL-6和TNF-α的变化特点,以探讨其在FS的病理生理学机制中的作用。方法:将大鼠随机分为:正常对照组(n=10,NC组),高热对照组(n=12,HC组)和热性惊厥组(n=21,FS组)。分别用ELISA和RT-PCR方法测大鼠海马IL-1β、IL-6和TNF-α蛋白和mRNA水平。结果:①FS组大鼠海马IL-1βmRNA和蛋白含量明显高于HC组(P<0.05或P<0.01)。FS组和HC组大鼠海马IL-6和TNF-α的mRNA及蛋白含量比较差别均无统计学意义(P>0.05)。②海马IL-6含量与海马IL-1β含量之间存在正相关关系(r=0.536,P<0.01,n=24)。结论:IL-1β参与了FS的病理生理学改变,IL-6和TNF-α与FS相关脑损伤的产生过程无关。

Objective：To analyze the pattern of the inflammatory response to frequent febrile seizures （FS） and pathophysiological mechanism of FS in an experimental rat FS model. Methods： Rats were divided into 3 groups： normal control group （n=10, NC group ）, hyperthermic control group （n=12, HC group） and febrile seizures group （n=21, FS group）. IL-1β ,IL-6 and TNF-α mRNA and protein levels in hippocampus of these rats were determined with RT-PCR and ELISA respectively. Results：①Significantly high level of IL-1β mRNA and protein in hippocamps were demonstrated in FS group compare with HC group（P〈0.05 or P〈0.01）. There were no significantly difference in hippocamps of IL- 6 and TNF-α mRNA /protein level between FS group and HC group （P〉0.05）.②Positive correlations were demonstrated between the level of IL-6 and IL-1β in hippocamps （r=0.536, P〈0.01, n=24）. Conclusion： IL-1β may play a role in the pathophysiology mechanism of FS, but IL-6 and TNF-α may have no relation with the development of FS induced brain damage.