外源Fe^(3+)诱导罗氏沼虾铁蛋白mRNA的表达

The expression of ferritin mRNA induced by iron in giant freshwater prawn Macrobrachium rosenbergii

探讨铁蛋白ferritin基因在罗氏沼虾中的组织表达分布及外源Fe3+对ferritin mRNA表达的影响。通过RT-PCR表明,ferritin mRNA在罗氏沼虾精巢、卵巢、肌肉、心脏、中肠腺和血淋巴中均有表达;荧光定量PCR结果证实了ferritin mRNA在中肠腺中表达量最高,其次是精巢。对罗氏沼虾进行FeCl3溶液肌肉注射后,荧光定量PCR结果表明,肌肉中ferritin mRNA表达量在注射后3 h增加了61倍,高水平的表达一直维持到8 h,随后在12 h表达量急剧地下降;卵巢中ferritin mRNA表达量在3 h有近4倍显著增加,在注射后48 h内一直维持在较高水平;心脏ferritin mRNA表达量在24 h后增加了8倍,48 h后表达量开始下降;在血淋巴、中肠腺和精巢注射前后均未发现有显著性差异(P>0.05)。以上实验结果表明,外源Fe3+可以诱导罗氏沼虾铁蛋白mRNA的表达。

Tissue expression of ferritin mRNA in giant freshwater prawn Macrobrachium rosenbergii was analyzed by RT-PCR.The results showed that ferritin transcript is widely expressed in testis,ovary,muscle,heart,hepatopancreas and hemocytes.Quantitative real-time PCR further revealed that the abundance of ferritin transcript is the highest in hepatopancreas,followed by testis.The expression of the ferritin transcript in the muscle significantly increased about 61-fold at 3h after injection of iron.This highly up-regulated expression was maintained till at 8 h after injection and thereafter sharply decreased to a lowlevel at12 h.In ovary,a significantly high expression was first detected with nearly 4-fold increase after 3 h of injection and then remained at higher level after 48 h of injection.In the heart,ferritin mRNA expression increased up to8-fold after 24 h and began to drop after 48 h.No significant difference （P〉0.05） was found in hemocytes hepatopancreas and testis.These data demonstrated that the expression of feritin in prawn can be regulated by iron at transcriptal level when insected.