食管鳞癌组织中RECK mRNA和蛋白的表达及意义

Expression of RECK mRNA and Protein in Esophageal Squamous Cell Carcinoma and Its Significance

目的:探讨食管鳞癌组织中RECK mRNA和蛋白的表达情况及其与临床病理因素的关系。方法:应用RT-PCR和免疫组化SP法检测62例食管鳞癌组织、31例癌旁不典型增生组织及62例正常食管粘膜组织中mRNA和蛋白的表达。结果:在食管鳞癌癌变过程中RECK在癌组织、癌旁不典型增生组织及正常粘膜组织中mRNA的含量依次增高,分别为1.052±0.078、1.274±0.235、1.306±0.121,组间比较有明显差异(F=49.936,P<0.05);不同分化程度、不同浸润深度及有无淋巴结转移的食管鳞癌组织之间RECK mRNA相对含量差异均有统计学意义(F=5.081,F=26.084,U=24.011,P均<0.05)。食管鳞癌组织及癌旁不典型增生组织中RECK蛋白表达均低于正常粘膜组织,表达量分别为59.7%(37/62)、71.0%(22/31)、85.5%(53/62),组间比较有明显差异(χ2=10.331,P<0.01);食管鳞癌组织中RECK蛋白表达与癌组织的分化程度、不同浸润深度及有无淋巴结转移密切相关(P<0.05)。结论:食管鳞癌组织中RECK mRNA和蛋白表达均降低,其低表达可能与食管鳞癌发生有关。检测RECK mRNA及蛋白的表达可望成为食管鳞癌早期诊断和判断预后的分子指标之一。

Objective： To examine the expression of RECK mRNA and protein in esophageal careinoma（EC）. Methods： RECK expression was detected by RT-PCR and SP immunohistochemical methods in 62 cases of EC tissue, 31 cases of mucosa adjacent to cancer, and 62 cases of normal esophageal mucosa. Results： The relative content of RECK mRNA was 1.052±0.078 in cancer tissue, 1.274±0.235 in mucosa adjacent to cancer and 1.306±0.121 in normal mucosa tissue, with a significant difference （F=49.936, P〈0.05） among the three types. The relative content of RECK mRNA was significantly relat- ed to the differentiation levels, depth of invasion and lymph node metastasis （F=5.081, F=26.084, U= 24.011, P〈0.05）. The positive rate of RECK protein expression was 59.7%（37/62） in cancer tissue, 71.0%（22/31） in mucosa adjacent to cancer and 85.5% （53/62） in normal rnucosa tissue. There was a significant difference among the three groups（P〈0.05）. There was a significant difference in RECK protein expression among different differentiation levels, depths of invasion and status of lymph node metastasis （P〈0. 05）. Conclusion： The expression of RECK mRNA and protein is lower in EC tissues, suggesting that the RECK gene may play a part in oncogenesis of EC. Detection of the expression of both RECK mRNA and protein may be used for early diagnosis and prognostic judgment of EC.