树头发菌丝体最适培养基的正交试验研究

Study on the optimum culture conditions of Pterula umbrinella Bres.mycelium by orthogonal experiment

采用四因素三水平的正交试验设计培养树头发菌丝体,对菌丝体直径进行测量及分析,探讨树头发菌丝体适宜的培养条件.结果表明:树头发菌丝体培养的各因素影响程度为培养基的pH值>蛋白胨>磷酸二氢钾>葡萄糖,最适培养条件为:葡萄糖15g/L,蛋白胨5.0 g/L,KH2PO4 0.25g/L,pH值5.8.

The optimum culture condition of Pterula umbrinella Bres. was investigated by the orthngonal experiment in four factors and three levels. The result showed that the order of the factors influencing mycelium growth was pH 〉 peptone 〉 KH2PO4〉 glucose and the optimum culture conditions were glucose 15g / L ,peptone 5.0 g/L, KH2PO4 0.25g / L, pH 5.8.