摘要
冷冻干燥是目前用于微生物、蛋白质等生物制品干燥保存的一种最常用、最有效的方法.介绍了冷冻干燥对细胞和蛋白质的影响,以及为消除这些影响所用的保护方式与保护剂,所涉及的保护剂包括多羟基化合物、糖、氨基酸、聚合物、蛋白质等;同时介绍了三种保护剂的作用机理即优先化作用、玻璃态假说和水代替价说及应用范围.
The method most commomlv used for store of proteins and microbial etc. is lyophilization. Theclassification, characters, and mechanism of denaturation protein on freezedry process are thorough discussed. To protect a protein from freezing and dehydration, a protector may be used. which are polyols, sugars, animo acids, polymers, proteins, and other protectors. There are primary three mechanisms of the protectors: the preferential interaction, the formation of an amorphous glass and the water replacement and application.
出处
《喀什师范学院学报》
2007年第6期46-50,共5页
Journal of Kashgar Teachers College
关键词
冷冻干燥
保护剂
机理
Lyophilization
Protectors
Mechanism