摘要
目的 探讨核转录因子-κB（NF-κB）、肿瘤坏死因子-α（TNF-α）和白介素-6（IL-6）在大鼠急性创伤性脑损伤后的表达及其变化规律。方法将实验Wistar大鼠随机分为两组：假手术组和脑损伤组。采用自由落体打击制造脑挫伤模型。损伤组分别于伤后6、24、72、120h处死动物，假手术组在术后6、72h分别处死。采用免疫组化，观察创伤性脑损伤后NF-κB、TNF-α表达的时序特点和ELISA方法测定血清IL-6含量变化特点。结果在假手术组动物的任何存活时间脑组织内均只能观察到很少的NF-κB、TNF-α的免疫反应，血清IL-6的含量低。在损伤动物的脑组织内，围绕损伤灶神经变性区，从伤后6-120h，可观察到逐渐增加的NF-κB、TNF-α的免疫反应，与假手术组比较差异有统计学意义（P〈0．05）。血清IL-6的含量与假手术组比较差异无统计学意义（P〉0．05）。结论创伤性脑损伤后NF-κB、TNF-α的激活是继发性脑损害的重要分子机制。IL-6未参与脑损伤后炎性反应。
Objective To investigate the expression ofNF-x B,tumor necrosis factor-alpha（TNF-α） and interleukin-6（IL-6） after traumatic brain injury. Methods The free-falling-body impact device was used to cause the models of local moderate contusion and laceration in the rats.Wistar rats weighing 250 - 300g were randomly divided into two groups：normal group（ n = 10） ,injury group（ n = 20） .The control group only received injuries and neither was given to the normal group. Rats of injury group were killed at 6,24,72, 120h after injury respectively.The injured brain tissue were fixed by paraformaldehyde and embedded paraffin and then sliced. Using immunohistochemistry in situ detection, A investigation was made to discover the relationship between the expression of NF-κB,TNF-α after traumatic brain injury and the secondary brain injury. IL-6 was inspected using ELISA. Results No immuno-reactivity for NF-κB,TNF-α was observed in normal group animals at any survival time. IL-6 was lower. In the neurodegenerative area surrounding the lesion of injury group, a significative increase in NF-κB and TNF-α immunoreactivity was observed from 6 - 120h. In contrast, inflammatory cells infiltration in trial group was effectively suppressed when the expression of NF-κB, TNF-α was significantly downregulated. Conclusion The study shows the activation of NF-κ B,TNF-α may be the important mechanism of acute traumatic brain injury with acute inflammation.
出处
《四川医学》
CAS
2007年第11期1254-1256,共3页
Sichuan Medical Journal
