摘要
提取人神经胶质瘤U87细胞总RNA,通过RT-PCR克隆到人基质金属蛋白酶2(MMP-2)类血红素结构域pex的cDNA序列-PEX,连接pMD18-T载体和PEX,测序分析,构建原核表达载体PET42a-PEX,转化大肠杆菌BL21(DE3),异丙基--βD-硫代半乳糖苷(IPTG)诱导后显示目的蛋白有37%的表达量,为进一步研究其抗肿瘤血管生成鉴定基础。
To clone the sequence of cDNA of hemopexin-like domain of human matrix metalloproteinase 2 （PEX） and construct its expression vector, the cDNA of PEX was amplified from human U87 glioma cells by RT-PCR, and subsequently cloned into pMD18-T vector for sequencing. Then the PEX was directed-cloned into PET-42a and construct the recombinant plasmid of PET42a-PEX. The recombinant PET42a-PEX vector was transformed into E. coli BL21（DE3） and the PEX expression was induced by IPTG. The expressed PEX accounted for approximately 37% of the total bacteria proteins.
出处
《西北农业学报》
CAS
CSCD
北大核心
2007年第6期94-96,106,共4页
Acta Agriculturae Boreali-occidentalis Sinica
基金
重庆三峡学院项目(0913601)
关键词
基质金属蛋白酶
PEX
抗血管生成
MMp-2
Hemopexin-like Domain
Angiogenesis
Cloning
Expression
