青海省马铃薯卷叶病毒的RT-PCR法检测

Detection of Potato Leafroll Virus by RT-PCR Amplification in Qinghai Province

根据马铃薯卷叶病毒(PLRV)外壳蛋白基因的保守序列设计了一对寡聚核苷酸引物,从田间自然感染PLRV的马铃薯病株中提取病毒总RNA,用反转录-聚合酶链式反应扩增出符合设计大小240bp的特异性产物,而对照没有任何扩增产物。建立了快速、准确检验PLRV的分子检测方法,为青海省马铃薯生产中卷叶病毒的检测和防治提供了有效手段。

A pair of DNA primers were designed and synthesized based on the nucleotide sequence of potato leafroll virus（PLRV）. The PLRV RNA which was used for cDNA synthesis was directly extracted from virus-infected potato leaves. A specific PCR fragment about 240 bp was obtained by reverse transcription and polymerase chain reaction（RT-PCR） amplification, which had the expected length of designed,at the same time, no fragment was obtained from the control. Therefore, a rapid and sensitive RT-PCR detection system of PLRV was established,which provided an effective way to detect and control PLRV in seeds potato product in Qinghai province.