裂盖马鞍菌甲醇提取物清除自由基活性的研究

Study on Free Radical Scavenging Activity of Methanol Extract from Helvella leucopus Pers.

采用DPPH法、水杨酸法、邻苯三酚自氧化法分别测定裂盖马鞍菌甲醇提取物清除DPPH自由基、羟自由基和超氧自由基的能力。结果表明,在实验条件下,裂盖马鞍菌甲醇提取物对超氧阴离子、羟自由基、DPPH自由基均有不同程度的抑制作用,最高清除活性分别达72.72%、54.90%、92.08%。因此裂盖马鞍菌甲醇提取物具有一定的体外抗氧化活性。

The methods of DPPH, salicylic acid and pyrograllol auto-oxidation were used to assay the scavenging quality of DPPH free radical, hydroxyl free radical（ · OH） and superoxide free radical（ · O2^-） in the methanol extract of Helvella leucopus Pets. in this article. The extract showed good scavenging effect on DPPH· , · OH and · O2^- under the condition of experiment, and the highest free radical scavenging rate of different free radical is 72.72%, 54. 90%, 92. 08% . So the methanol extract of H. leucopus Pets. showed a certain extent antioxidant effect in vitro .