应用rLF检测人群血清抗炭疽抗体水平的初步评价

Preliminary evaluation on the detection of serum level of anti-anthrax antibodies in population by using the recombinant lethal factor protein

目的对重组炭疽致死因子(rLF)在人群血清抗体水平检测中的初步应用进行评价。方法间接ELISA方法检测人群血清特异抗体,检测结果按照抗体相对含量进行分类比较、t检验、S/N比值大于等于2.1的阳性判断标准等多种方法进行统计分析。结果rLF的检测结果能区别大部分的病人和健康人,实验结果还显示出在我国部分地区的健康人尤其是健康从业人员中也有相当一部分人抗体水平较高,有的甚至达到了感染病人的抗体水平,提示炭疽在我国部分地区有较强隐性感染。结论致死因子显示出一定的应用潜力,可能用在炭疽的临床诊断、疾病监测、以及用来评估某地区的炭疽流行强度。

To evaluate the feasibility of the recombinant lethal factor protein（rLF）applied to the detection of serum antibody level in population for anthrax surveillance,the anti-anthrax antibody level in population was determined by indirect ELISA and the statistical analysis consisting of the comparison of relative concentrations of antibodies,t-test and the cut-off value on S/N ratio≥2.1.It was found that the use of rLF to detect the levels of serum anti-anthrax antibodies in population could distinguish effectively most of patients with anthrax and healthy population.The experimental results also revealed that most of the healthy individuals in certain area of our country,especially those with occupational work at risk,showed high level of anti-rLF antibody,and some of them even might attain to the level of antibodies in infected patients,indicating the higher percentage of latent infection with anthrax in certain area of China.From these observation it is clear that rLF shows certain potential for practical application and its use is valuable in clinical diagnosis,disease surveillance and to evaluate the intensity of epidemiogenesis of anthrax in some area.