苏云金杆菌δ-内毒素对离体昆虫细胞作用机制的研究

MODE OF ACTION OF BACILLUS THURINGENSIS δ ENDOTOXIN ON INSECT CELLS IN VITRO

利用ＢａｃｉｌｕｓｔｈｕｒｉｎｇｉｅｎｉｓｉｓＨＤ－１δ－内毒素作用离体培养的昆虫细胞（Ｐｘ），并测定其对Ｐｘ细胞葡萄糖、Ｎａ＋、Ｋ＋离子吸收的影响．实验表明，δ－内毒素作用Ｐｘ细胞后０．５ｍｉｎ内，细胞对葡萄糖的吸收大量增加，１０ｍｉｎ后，葡萄糖的吸收基本停止，细胞内Ｎａ＋含量的快速增加发生在δ－内毒素作用５ｍｉｎ后，Ｋ＋离子的含量在１ｍｉｎ后开始增加，但增加缓慢．

The experiment results showed that: being treated with B.t. δ endotoxin, Px cells started to absorb glucose rapidly in no less than 0.5 min, and 10 more min late, Px cell sstopped uptaking glucose. The δ endotoxin, however, did not cause accumulation of Na + ion in the first 5 min and K + ion in the first 1 min in Px cell. 1 min after the treatment with B.t. δ endotoxin, the amount of K + ion in Px cells increased slowly and 5 min after that, the amount of Na + ion in Px cells increased rapidly. All above showed that δ endotoxin first made impact on the influs of glucose and them the uptaking of Na +,K + ion on Px cells.