Connexin37基因C1019T多态性与冠心病的关联研究

The Association between Connexin37 Gene C1019T Polymorphism and Coronary Heart Disease.

目的研究缝隙连接蛋白Connexin37基因C1019T多态性与冠心病危险性的关系。方法采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法检测了173例冠心病患者和148例对照的Connexin37基因C1019T基因型和等位基因的分布。结果两组基因型频率符合Hardy-Weinberg平衡。冠心病组的Connexin37基因TT和TC基因型的频率显著高于对照组(54.9%比39.2%,P<0.05),同样冠心病组T等位基因的频率显著高于对照组(35.3%比23.6%,P<0.01)。经Logistic回归分析,T等位基因携带者(TT+TC)患冠心病的危险性是CC基因型的2.34倍(95%的CI为1.03-5.32,P<0.05)。结论Connexin37基因C1019T多态性与冠心病的危险性相关,其中T等位基因可能是冠心病的遗传危险因素。

Objective To investigate the association between gap junction protein Connexin37 gene C1019T polymorphism and corenary heart disease （CHD）. Methods Using polymerase chain reaction - restrictive fragment length polymorphism （PCR-RFLP） method, the genotype and allele distribution of Connexin37 gene C1019T polymorphism in 173 CHD patients and 148 controls were analyzed. Results Distribution of Connexin37 genotype was in Hardy-Weinberg equilibrium for both groups （CHD and control groups）. The frequency of TT and TC genotype in CHD group was significandy higher than in control group （54.9% vs 39.2%, P 〈 0.05） ; similarly, the frequency of T allele in CHD group was significantly higher than in control group （35.3 % vs 23.6 %, P 〈 0.01）. After adjusted for the influence of age, gender and other CHD risk factors by multiple Logistic regression analysis, those who are T allele carriers （TT ＋ TC） have as 2.34 times higher risk to suffer from CHD than those who have CC genotype （95%CI = 1.03 - 5.32, P 〈 0.05）. Conclusion Connexin37 gene C1019T polymorphism is associated with CHD risk, T allele carriers have a higher risk to suffer from CHD, T allele may serve as a genetic risk factor of coronary heart disease.