乳胶凝集试验快速诊断轮状病毒的临床应用

The clinical application of latex agglutination in the rapid diagnosis of Rotavirus

目的研究轮状病毒快速诊断法的临床应用。方法用轮状病毒的快速鉴定试剂盒对153例1~7岁患儿的大便标本,做玻片法乳胶凝集试验,进行轮状病毒致小儿腹泻、胃肠炎的快速诊断。结果轮状病毒致小儿腹泻的阳性检出率为46.25%（37/80）,致小儿胃肠炎的阳性检出率为38.36%（28/73）,总阳性率为42.48%（65/153）。皆于2min内出结果。阳性结果中未发现交叉反应,亦符合临床诊断。结论该快速诊断法具有操作简便、快速、准确、特异性强、阳性率高等特点。可以在基层和农村医疗单位推广应用,它为诊断治疗轮状病毒致腹泻、胃肠炎提供了及时准确的客观依据。

Objective： To research into the clinical application of the rapid diagnosis of Rotavirus. Methods ： A rapid diagnosis of fecal specimens take from the 153 cases 1 - 7 years old children was successfully determined by the kit for rapid identification of Rotavirus cause of agdiarrhoea and gastroenteristis using, the Rotavlrus latex agglutination test. Results： The positive detecting rates of diarrhoea and gastroenteristis patients by infection Rotavirus were 46.25% （37/80）, 38.36 （28/73）. Total positive rate of the 153 cases was 42.48% （65/153）. These results of test were obtained within two minutes. In the positive results, none of the positive cases showed cross agglutination phenomena, completely identified with clinical diagnosis. Conclusion： The procedures involved in the test are simple, the diagnosis in fast, and the result is high accurate and specific. This method is particularly suitable for the use in rural hospital.