耐药鲍曼不动杆菌扩增片段长度多态性分析指纹图谱数据库的建立

Establishment of fingerprint database for drug-resistant strain of Acinetobacter baumannii by AFLP method

目的:建立鲍曼不动杆菌扩增片段长度多态性分析(AFLP)指纹图谱数据库。方法:采用NCCLS 2004年版抗生素敏感性判断方法研究药物敏感性,采用AFLP技术研究鲍曼不动杆菌基因型,以AFLP结果建指纹图谱立数据库。结果:药敏结果和AFLP指纹图谱数据库进行的聚合分析结果具有一致性。结论:AFLP指纹图谱数据库具有实用价值。

Objective. To establish fingerprint database of drug-resistant strain of Acinetobacter baumannii with AFLP genotype. Methods. Twenty-seven strains of A. baumannii were clinically isolated. The microbial sensitivity to 13 antibiotics was analyzed according to NCCLS 2004. The genotype was analyzed by amplified fragment length polymorphism （AFLP） method. Results. The results were consistent with those with AFLP database. Conclusion; The database of AFLP fingerprint may have practical value for identification of drug-resistant Acinetobacter baumannii.