8-(2′-萘磺酰)-胺基喹啉锌荧光探针的合成及其对酵母细胞的染色

The Synthesis of One Zinc Fluorescence Probe Based on N-(8-quinolyl)-β-naphthalene Sulfonamide and the Stain of Yeast Cell

合成了一种锌离子荧光探针-8-(2′-萘磺酰)-胺基喹啉(NSQ),NSQ自身具有较弱的荧光,向NSQ溶液中加入Zn2+后,荧光增强5.7倍,并且生物体系中含量较多的碱金属或碱土金属离子几乎不干扰Zn2+的荧光.酵母细胞染色实验表明,NSQ可以对酵母细胞中的锌离子进行荧光显微分析.

N-（8-quinolyl） -β-naphthalene sulfonamide （NSQ） was synthesized. NSQ exhibited a high binding affinity and good fluorescence sensing ability to Zn^2＋. Under the same conditions, several metal ions commonly present in a physiological environment, such as Na^＋ , K^＋ , Ca^2＋ , Mg^2＋ , Mn^2＋, Fe^2＋, and Co^2＋, showed little interference to the fluorescence response to Zn^2＋. The cell staining experiments showed that NSQ could be used for the imaging of Zn^2＋ in yeast cells.