转染γ-干扰素的口腔黏膜细胞片修复裸露硬腭对上颌骨腭突生长发育的影响

To study the effect of oral mucosa cell transfected with IFN-γ recover denuded hard palate to the growth of maxilla

目的探讨转染γ-干扰素的口腔黏膜细胞片修复裸露硬腭,对预防上颌骨腭突生长受限的作用,以便最大限度的减少腭裂术后上颌骨腭突继发畸形的发生。方法3周龄雌性SD大鼠80只,左侧硬腭裸露后,随机分成裸露面自行修复（Ⅰ）组、羊膜修复（Ⅱ）组、羊膜负载的口腔黏膜干细胞片修复（Ⅲ）组、羊膜负载的转染γ-干扰素基因的口腔黏膜干细胞片修复（Ⅳ）组等4种不同的处理方法组,每组20只。术后9周测量硬腭左右侧宽度,并采用SPSS11.0统计软件对数据进行处理,分析不同处理方法对硬腭宽度的影响。结果Ⅰ、Ⅱ、Ⅲ、Ⅳ组硬腭非对称率均数分别为（68.64±9.03）%、（58.53±7.40）%、（53.12±4.92）%、（52.25±4.61）%,Ⅰ组与Ⅱ、Ⅲ、Ⅳ组间比较有差异均有统计学意义（P〈0.05）,Ⅱ组与Ⅲ、Ⅳ组间比较差异有统计学意义（P〈0.05）,而Ⅲ组与Ⅳ组间比较差异无统计学意义（P〉0.05）。结论羊膜与组织工程化的口腔黏膜修复裸露硬腭的方法对预防上颌骨的继发畸形有积极的意义,组织工程化的口腔黏膜法优于单纯应用羊膜进行修复组,转染γ-干扰素的组织工程化口腔黏膜优于未转染组。

Objective To study the effect of oral mucosa cell transfected with IFN-γ recover denuded hard palate to the growth of maxilla in rats. Methods Divide 3-week-age 80 female rats with left denuded hard palate into 4 groups randomly, depends on different methods recovered the denuded hard palate, granulation tissue growing itselves（ Ⅰ ）, transplanting amnion（ Ⅱ ）, transplanting oral mucous cell loaded by amnion（ Ⅲ）, or oral mucous cell transfected with IFN-γ （ Ⅳ）, 20 rats in one group. After 9 weeks, measure the width of left hard palate and the right, and analyse the width of hard palate by different methods. Results The antisymmetry rate of hard palate width of groups Ⅰ , Ⅱ, m and Ⅳare（68.64 ±9.03）%, （58.53 ± 7.40）%, （53.12 ±4.92） % and （52.25 ± 4.61 ）%, statistical analysis through SNK of SPSS11.0 show group I is different from groups Ⅱ , Ⅲ , Ⅳ（ P 〈 0.05 ）, group Ⅱis different from groups m , Ⅳ（ P 〈 0.05）, but group m is not different from group Ⅳ（ P 〉 0.05）. Conclusions It has energetic significance to prevent maxillary secondary deformity with amnion and tissue-engineered oral mucosa recover denuded hard palate, the tissue-engineered oral mucosa is better than amnion, the tissue-engineered oral mucosa transfected with IFN-γ is better than not transfected.